Fig. 6

Nicotinic AChR ligands inhibit RANKL-induced Ca²⁺ oscillation. a, b Intracellular Ca²⁺ level in BMMs, a cultured in the presence of M-CSF for 72 hours and b cultured in the presence of M-CSF and RANKL for 72 hours. c–g Effect of acute administration of nAChR ligands on intracellular Ca²⁺ level in BMMs cultured in the presence of M-CSF and RANKL for 72 hours. c Nicotine (Nic; 100 μg/ml); d PNU282987 (PNU; 5–10 μM); e acetylcholine (Ach; 10 μM); f alpha-bungarotoxin (BTX: 100 nM); g mecamylamine (Mec; 10 μM) and ACh (10 μM). h Intracellular Ca²⁺ levels in BMMs cultured in the presence of M-CSF, RANKL and nicotine (100 μg/mL) for 72 hours