Fig. 5

Anti-colony stimulating factor-1 receptor (anti-CSFR1) antibody reduces the production of inflammatory mediators in synovial tissue in rheumatoid arthritis (RA). a IL-6 ELISA production in supernatants of RA synovial tissue after 24-h incubation in medium alone or increasing concentrations of colony stimulating factor-1 (CSF-1) or IL-34 (n = 7). b-d IL-6 ELISA production in supernatants of RA synovial tissue after 4 days of incubation in medium alone in the presence of IgG1 or anti-IL-34 antibody (Ab) (5 μg/ml for both, n = 5) (b), anti-CSF-1 Ab (5 μg/ml, n = 3) (c), or increasing concentrations of IgG4 or huAB1 (n = 5) (d). e, f Multiplex analysis of protein production in supernatants of RA synovial tissue after 4 days of incubation in medium alone in the presence of 1 μg/ml IgG4 or 1 μg/ml huAB1 (n = 4). Boxes represent the 25th–75th percentiles, lines within the boxes mark the median value, and lines outside the boxes denote the 10th and 90th percentiles. *P < 0.05 and **P < 0.01