Fig. 2

Embryonic stem cell-derived mesenchymal stromal cells (hESC-MSC) administration alters the regulatory T cell (Treg)/effector T cell (Teff) balance in mice with collagen-induced arthritis. The frequency of Treg and Teff cells was analyzed by flow cytometry in the inguinal lymph nodes (ILN) 10 days after arthritis onset in mice with established disease. a Representative flow cytometry plots and quantification of CD4 Treg and Teff cell frequency in hESC-MSC-treated and control mice. b Treg/T helper (Th)17 ratio is significantly increased in hESC-MSC-treated mice compared to the control group. Data shown are values from individual mice and the mean of experimental groups. Statistical differences were analyzed by the Mann-Whitney U test. *P < 0.05; **P < 0.01. PBS phosphate-buffered saline, IFN interferon