Skip to main content
Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: HM71224, a novel Bruton’s tyrosine kinase inhibitor, suppresses B cell and monocyte activation and ameliorates arthritis in a mouse model: a potential drug for rheumatoid arthritis

Fig. 4

HM71224 inhibits cytokine production by human peripheral blood monocytes and plasmacytoid dendritic cells. a Human CD14+ monocytes were isolated from healthy donors (n = 3) and stimulated with heat-inactivated immune complexes (ICs) in the presence of HM71224. The levels of the indicated cytokines in the culture supernatant were then measured by ELISA at 5 h post stimulation. HM71224 significantly reduced the levels of secreted cytokines in a dose-dependent manner. b Human peripheral blood dendritic cells were isolated from healthy donors (n = 2) and stimulated with CpG oligodeoxynucleotides (CpG ODN) or lipopolysaccharide (LPS). The levels of the indicated cytokines in the culture supernatant were then measured by ELISA at 48 h post stimulation.*P < 0.05, **P < 0.01, and ***P < 0.001 vs. no treatment. LPS lipopolysaccharide

Back to article page

Arthritis Research & Therapy

ISSN: 1478-6362

Contact us