Fig. 5

E2 stimulation activates human miR-140 promoter activity and is mediated by the −336/-321- and −306/-318- bp sequences. a −495 proximal region sequence upstream of the miR-140 star sequence contains two classical estrogen response elements with the consensus sequence 5′-GGTCAnnnTGACC-3′. b Luciferase activity in SW1353 cells transfected with miR-140 promoter wide-type (pGL3.-pmiR-140) and two miR-140 promoter mutants (mut −336/-321 and mut −306/-318), 12 hr after transfection, the cells were serum starved for 12 hr followed by 4-hr treatment with E2 (10 nM). The date are expressed as mean and SEM of three independent experiment. ^*Statistically different from control (P < 0.05). ERE estrogen response element, TSS transcription start site