Fig. 3

Anti-CII IgG and IgM in serum from LNT-Igk-Ctrl and LNT-Igk-CII mice. Serum levels of a anti-CII IgG, b anti-CII IgG1, c anti-CII IgG2a, d anti-CII IgG2b and e anti-CII IgM in LNT-Igk-Ctrl and LNT-Igk-CII mice measured by ELISA at day 20, day 40 (n = 10 per group) and day 55 (n = 6 per group) after immunization. f Change in serum levels of the IgG antibodies to the specific CII epitopes (C1, J1, D3, U1, E10 and F4) measured as Δabsorbance (absorbance at indicated day – mean value at day 0). Serum was obtained from LNT-Igk-Ctrl and LNT-Igk-CII mice at day 0 (n = 3 per group), day 15 (n = 6 per group), day 40 (n = 10 per group) and day 55 (n = 7 and 6, respectively). Data shown as mean ± SEM. Indicated P values were determined using a two-tailed t test with a Bonferroni correction for multiple comparisons when comparisons were made for multiple anti-recombinant CII-peptide assays. *P < 0.05; **P < 0.01; ***P < 0.001