Group | Polybacterial infection | Positive systemic tissue samples |
---|
Heart | Liver | Spleen | Kidney | Lung | Knee joint |
---|
n = 10 |
n = 10 |
n = 5 |
n = 10 |
n = 10 |
n = 10 |
---|
I |
Pg/Td/Tf
| 4/3/1 | 2/3/1 | 0/0/0 | 5/4/2 | 2/0/0 | 0/0/0 |
II |
Pg/Td/Tf + collagen | 5/2/0 | 3/2/1 | 0/0/0 | 4/5/2 | 3/1/0 | 2/0/0 |
III | Collagen control | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 |
IV | Sham infection | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 |
- After 24Â weeks of gingival infection, heart, liver, spleen, kidney, lung, and hind limb ankle joint tissues were harvested from B10.RIII mice and extracted genomic DNA were subjected to 40Â cycles of PCR analysis using species-specific primers for the three oral bacteria P. gingivalis (Pg), T. denticola (Td), and T. forsythia (Tf). The numbers indicated with forward slash correspond to the number of mice positive for Pg/Td/Tf genomic DNA, respectively