Fig. 6

Phenotypic characterization of GFP⁺ cells in synovium. a Paraffin-embedded sections from control and injured knee joints at 7 days post-injury (7dpi) and 4 weeks post-injury (4wpi) were subjected to immunofluorescence staining for GFP (green) in conjunction with CD16/CD32 (red) or Pdgfrα (red) and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bars = 25 μm. The insets show magnified views of the areas within dotted boxes. S synovium, C capsule, P patella, F femur. b, c GFP⁺CD16/CD32⁺ cells and GFP⁺Pdgfrα⁺ cells in the synovium shown as percentages of (b) the total GFP⁺ cell population and (c) the total marker-positive cell population. Data are shown as individual data points from three mice, with horizontal lines indicating mean values. GFP green fluorescent protein, Pdgfrα platelet-derived growth factor receptor α