Table 1 UPR genes having 1.3-fold change expression in human chondrocytes as determined by PCR array*
Increased expression | Decreased expression | ||
|---|---|---|---|
A) Osteoarthritic chondrocytes (n = 3) compared to normal chondrocytes (n = 3) | |||
SREBF2 | 4.71 | CEBPB | -1.98 |
ERN2 | 1.81 | UBE2G2 | -1.87 |
ATXN3 | 1.61 | HTRA4 | -1.84 |
SCAP | 1.57 | NPLOC4 | -1.73 |
SREBF1 | 1.52 | ERN1 | -1.71 |
PDIA3 | 1.49 | DNAJB9 | -1.60 |
ERO1L | 1.48 | GRP78 (HSPA5) | -1.59 |
HSPA4L | 1.46 | INSIG1 | -1.51 |
DNAJB2 | 1.45 | XBP1 | -1.38 |
EDEM3 | 1.45 | HTRA2 | -1.32 |
SEC62 | 1.44 | Â | Â |
NUCB1 | 1.40 | Â | Â |
GANC | 1.39 | Â | Â |
SYVN1 | 1.35 | Â | Â |
BAX | 1.34 | Â | Â |
INSIG2 | 1.33 | Â | Â |
B) Osteoarthritic chondrocytes (n = 3) treated with thapsigargin (50 nM) compared to untreated (control) chondrocytes | |||
GRP78 (HSPA5) | 26.01 | PRKCSH | -4.37 |
CHOP (DDIT3) | 20.76 | SREBF1 | -4.18 |
HERPUD1 | 16.88 | MAPK10 | -3.58 |
DNAJB9 | 14.39 | HSPA2 | -2.66 |
ERO1LB | 13.90 | PPIA | -2.57 |
MANF | 8.69 | HSPA4L | -2.18 |
PPP1R15A | 6.98 | FBXO6 | -2.11 |
SEL1L | 6.76 | HSPA1L | -1.98 |
ERN1 (IRE1) | 6.10 | PFDN5 | -1.91 |
SYVN1 | 5.99 | MAPK9 | -1.84 |
SERP1 | 4.98 | GANC | -1.81 |
EDEM1 | 4.55 | HTRA4 | -1.77 |
SELS | 4.49 | CCT7 | -1.75 |
PERK (EIF2AK3) | 4.15 | HSPA1B | -1.71 |
DERL2 | 3.90 | INSIG1 | -1.70 |
DNAJC3 | 3.67 | NPLOC4 | -1.64 |
PDIA3 | 2.81 | BAX | -1.62 |
SEC63 | 2.54 | HSPA4 | -1.58 |
DNAJC10 | 2.48 | DNAJB2 | -1.57 |
DERL1 | 2.27 | TOR1A | -1.55 |
ATF4 | 2.17 | UBE2G2 | -1.51 |
CALR | 2.13 | MBTPS1 | -1.45 |
XBP1 | 1.98 | GANAB | -1.43 |
ERP44 | 1.89 | SREBF2 | -1.39 |
ERO1L | 1.75 | ERN2 | -1.36 |
VCP | 1.72 | HSPH1 | -1.35 |
UGGT1 | 1.60 | AMFR | -1.35 |
ATF6 | 1.54 | SIL1 | -1.31 |
OS9 | 1.52 | ATF6B | -1.30 |
CANX | 1.52 | Â | Â |
RPN1 | 1.42 | Â | Â |
RNF5 | 1.34 | Â | Â |
C) Osteoarthritic chondrocytes (n = 3) treated with tunicamycin (500 ng/ml) compared to untreated (control) chondrocytes | |||
GRP78 (HSPA5) | 21.89 | SREBF1 | -3.79 |
CHOP (DDIT3) | 15.69 | INSIG1 | -3.17 |
HERPUD1 | 11.67 | HSPA2 | -2.77 |
ERO1LB | 10.34 | PPIA | -2.27 |
DNAJB9 | 9.44 | HSPA4L | -2.03 |
MANF | 6.76 | PRKCSH | -1.97 |
SEL1L | 5.66 | HTRA4 | -1.95 |
ERN1 (IRE1) | 4.57 | SREBF2 | -1.94 |
SERP1 | 3.94 | FBXO6 | -1.91 |
PPP1R15A | 3.75 | NPLOC4 | -1.74 |
DNAJC3 | 3.67 | MAPK10 | -1.70 |
SYVN1 | 3.58 | MAPK9 | -1.63 |
EDEM1 | 3.53 | CCT7 | -1.62 |
SELS | 3.34 | PFDN5 | -1.61 |
DERL2 | 3.04 | HSPA1B | -1.60 |
PERK (EIF2AK3) | 2.27 | HSPH1 | -1.59 |
ATF4 | 2.20 | GANC | -1.57 |
PDIA3 | 2.09 | ERN2 | -1.47 |
SEC63 | 2.03 | HSPA4 | -1.46 |
DNAJC10 | 2.01 | TOR1A | -1.46 |
CALR | 1.86 | INSIG2 | -1.41 |
DERL1 | 1.80 | UDF1L | -1.40 |
ERP44 | 1.59 | TCP1 | -1.39 |
UGGT1 | 1.58 | MBTPS1 | -1.35 |
XBP1 | 1.47 | Â | Â |
ATF6 | 1.45 | Â | Â |
CANX | 1.37 | Â | Â |