Fig. 3From: Active but not inactive granulomatosis with polyangiitis is associated with decreased and phenotypically and functionally altered CD56dim natural killer cellsCD56dim but not CD56bright natural killer (NK) cells are reduced in active (non-remission) granulomatosis with polyangiitis (GPA). Peripheral blood mononuclear cells from patients with GPA (totalā=ā19, in remissionā=ā11 and non-remissionā=ā8 patients) and 9 healthy controls (HC) were isolated by density gradient centrifugation and analyzed by multicolor flow cytometry (panel III). a Gating strategy. Peripheral blood lymphocytes were determined using forward scatter (FSC) and side scatter (SSC). Cell viability was assured by negative Zombie aqua staining. NK cells were determined by co-staining of CD3 (negative) and CD56 (positive). b Percentages of CD56dim (left) and CD56bright (right) NK cells among total NK cells were plotted according to activity states. The Kruskal-Wallis test was significant in both graphs (pā=ā0.0084 and pā=ā0.0081), Dunn's post hoc test was significant where indicated in the graphs; bars represent medians. c Absolute numbers of CD56dim (left) and CD56bright (right) NK cells were calculated on the basis of the absolute NK cell counts shown in Fig.Ā 1. The Mann-Whitney test was significant only for CD56dim NK cells (pā=ā0.0149); bars indicate medians. ns not significantBack to article page