Fig. 3

Regulation of nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) transcriptional activity by the I473T variant. a Three-dimensional structure of Toll-like receptor 10 (TLR10) visualized by using the Jmol viewer showing the location of residue I473. NAG 2-(acetylamino)-2-deoxy-β-d-glucopyranose. b and c K562 and U937 cells were cotransfected with wild-type (wt) TLR10 or its mutated variant (mut) and a reporter vector containing NFkB-responsive sequences. Cells were stimulated with 10 ng/ml tumor necrosis factor-α (TNFα) for 24 h, and then cell extracts were prepared and analyzed for luciferase activity. d and e K562 and U937 cells transfected with the indicated TLR10 variants were treated with TNFα in the presence or absence of infliximab, and luciferase activity was analyzed. Histograms show the mean + SD of three independent experiments. *p < 0.05