Fig. 4

Regulation of the levels of nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) target genes by the I473T variant. U937 and K562 cells were transfected with Toll-like receptor 10 (TLR10) (wild type [wt] or mutated variant [mut]) and then treated or not with 20 ng/ml tumor necrosis factor-α (TNFα) for 24 h. The expression of NFkB target genes chemokine (C-C motif) ligand 2 (CCL2) (a, d), TNF-related apoptosis-inducing ligand (TRAIL) (b, e), and TNFα (c) were determined by performing quantitative real-time polymerase chain reactions. f Cells transfected with the indicated variants were treated with TNFα in the presence or absence of infliximab, and, 24 h later, the messenger RNA (mRNA) levels of CCL2 were determined. β-actin was used for normalization. C Cells transfected with empty vector as an additional control. Histograms show the mean ± SD of three independent experiments. *p < 0.05