Fig. 5

Fibrotic responses are negatively regulated by cellular A20. Foreskin fibroblasts were transfected with A20-specific siRNA or scrambled (Scr) control, followed by incubation with transforming growth factor-ß2 (TGF-ß2) (10 ng/ml), lipopolysaccharide (LPS) (500 ng/ml) or both for 24 h. a, b mRNA levels were determined by real-time qPCR. Results, normalized with glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are means ± SD of triplicate determinations (n = 2). c Western analysis of secreted Type I collagen (Cgn I). ns nonspecific band