Fig. 1

Th17 response to oral challenge with live P. gingivalis. Healthy suspensions of live P. gingivalis were mixed with CMC and brushed onto the gingival tissues of B6.DR1 mice (shaded pink area). a Contour plots for flow cytometric analysis of cervical lymph nodes and peripheral blood mononuclear cells from individual B6.DR1 mice with or without treatment with P. gingivalis. Plots are gated on CD3⁺CD4⁺ T cells. Red numbers in each quadrant represent the percentage of CD3⁺CD4⁺ T cells expressing the IL-17F^mrfp reporter, green numbers represent the percentage of CD3⁺CD4⁺ T cells expressing the Foxp3^gfp reporter. b Compilation of Th17 data over the course of 2 months after treatment. Results are expressed as mean percentage of CD3⁺CD4⁺ T cells expressing IL-17F^mrfp. N = 3 or 4 mice at each time point. Error bars = SEM. GFP green fluorescent protein, mRFP mono-red fluorescent protein (Color figure online)