Fig. 5

Reduction of infiltrating, autoreactive T cells in the lungs of MRL/lpr mice treated with high-dose irinotecan (Irino). Lungs were harvested from 18-week-old MRL/lpr mice that received saline (n = 7), 25 mg/kg irinotecan (n = 6), or 1 mg/kg irinotecan (n = 6). MRL/MpJ mice treated with saline (n = 7) were used as controls (Ctrl). a Lung cells were analyzed by flow cytometry for B cells (CD3^–B220⁺CD138^–) and T-cell subsets (CD4⁺, CD8⁺, and DN). b Total numbers of IgG-secreting cells isolated from the lungs were determined by enzyme-linked immunosorbent spot assay. In (a), symbols represent individual mice; bars show mean ± SEM.*P < 0.05, **P < 0.01, ***P < 0.001, versus saline-treated MRL/lpr mice by one-way analysis of variance corrected with Bonferroni’s multiple comparisons test