Fig. 5

In vivo cartilage destruction and vascular transmigration following implantation of RASF under the kidney capsule of SCID mice. a Representative confocal images of tissue sections of the fibro/cartilage matrix harvested 60 days following implantation showing expression of CD248 and podoplanin (PDPN). Dashed line shows demarcation of the cartilage interface. Scale bar = 100 μm. b The percentage of cells positive for each marker within 20 μm proximity to cartilage is displayed as the mean ± SEM for each cell marker. Time course analysis of the percentage of total nucleated cells expressing PDPN (c) and CD248 (d) within the primary implant compared to the secondary (cartilage only) implant at 6, 12, 18, 30, and 60 days following implantation. Absolute numbers of total nucleated cells over time in sections of secondary implant are also shown compared to the total number of those cells positive for each of these markers over time (e, f). Data are expressed as the mean ± SEM of the percentage of cells expressing each cell marker at each time point from eight tissue sections per implant (n = 3 mice). **P < 0.001 . C cartilage fragment