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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout

Fig. 4

Recombinant human 1 L-37 (rhIL-37) reduces inflammatory cell recruitment in mice with monosodium (MSU) crystals induced peritonitis. ac Neutrophil, monocyte and macrophage determination by flow cytometry: neutrophils are defined as Gr-1high7/4+ (gate R1); monocytes are defined as Gr-1inter7/4+ (gate R2) expressing low levels of F4/80 (gate R3); macrophages with high expression of F4/80 (gate R4) are thought to be resident macrophages. d Total and separate proportions of neutrophils and monocytes in all peritoneal exudate cells; **P < 0.01,***P < 0.001. e Concentration of neutrophil chemoattractant in peritoneal lavage fluid; ***P < 0.001. f Concentration of monocyte chemoattractant in peritoneal lavage fluid; **P < 0.01. g Different dosage of rhIL-37 was given preventively or therapeutically in mice with gouty arthritis, and foot thickness was evaluated; **P < 0.01, ***P < 0.001. h, i Histopathological analysis of joint from non-rhIL-37 treatment group by H&E staining (×100 original magnification (h) and × 200 original magnification (i)); arrow abundant inflammation in soft tissue and joint space. j Histopathological analysis of joint from rhIL-37 preventive group by H&E staining (×100 original magnification); arrow less inflammation in soft tissue and joint space. k, l Histopathological analysis of joint from rhIL-37 therapeutic group by H&E staining (×100 original magnification (k) and × 200 original magnification (l); arrow almost no inflammation in soft tissue and joint space

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