Fig. 2

T cell Ig and mucin domain protein 1 (TIM-1) expression upon B cell receptor (BCR) and Toll-like receptor-9 (TLR9) activation in healthy subjects and patients with systemic sclerosis (SSc). a. a Representative histograms (left) and summarizing graph (right) show the percentage of TIM-1-expressing B cells from healthy subjects in different subpopulations, left unstimulated or activated with an anti-BCR antibody (αBCR) and CpG for 48 hours. b Percentage of TIM-1⁺ total, transitional, naïve and memory B cells from healthy controls and patients with SSc after stimulation with αBCR and CpG for 48 hours. c Expression levels of TIM-1 on unstimulated (left) or stimulated (right) transitional B cells from patients with SSc with the diffuse or limited form of the disease. d Correlation between the expression levels of TIM-1 in stimulated transitional B cells from patients with SSc and the diffusing capacity for carbon monoxide (DLCO). e. Correlation between the expression levels of TIM-1 in unstimulated (left) or stimulated (right) transitional B cells from patients with SSc and the Rodnan score of skin involvement. MFI mean fluorescence intensity. *P < 0.05, **P < 0.01