Fig. 3

Immunohistochemical staining. Lung sections from an early lung cancer patient (a–d), a surviving patient with classic dermatomyositis (DM)-related interstitial lung disease (ILD) (e–h: 66 year-old male patient, serum sCD163 = 519 ng/mL), and a non-surviving patient with classic DM-related ILD (i–l: 32 year-old male patient, serum sCD163 = 1572 ng/mL) are shown. CD163-positive macrophages are evident in the lungs of the two patients with classic DM-related ILD, especially in lung of the non-survivor. Accumulations of CD163-positive alveolar macrophages are observed (arrows). a, e, and i Hematoxylin − eosin staining; scale bar 200 μm. b, f, and j Isotype controls, scale bar 200 μm. c, g, and k Staining with anti-CD163 antibody with lower magnification (×40), scale bar 200 μm. d, h, and l Staining with anti-CD163 antibody with higher magnification (×100), scale bar 100 μm