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Table 7 Skeletal muscle metabolic intermediate concentrations

From: Molecular alterations in skeletal muscle in rheumatoid arthritis are related to disease activity, physical inactivity, and disability

 

Rheumatoid arthririts (n = 51)

Controls (n = 50)

Mean

SD

Mean

SD

Amino acids

 Glycine

1012.669

304.568

1042.875

360.089

 Alanine

2781.241

876.247

2735.464

820.802

 Serine

773.987

190.246

777.726

286.455

 Proline

502.861

179.5

528.023

222.984

 Valine

291.82

75.004

300.739

99.003

 Leucine/isoleucine

659.544

197.996

663.116

233.404

 Methionine

54.167

14.36

55.372

17.48

 Histidine

488.821

164.605

548.187

276.048

 Phenylalanine

77.739

22.414

80.155

27.759

 Tyrosine

80.962

23.249

88.815

32.052

 Aspartate/asparagine

100.518

62.088

144.672

198.704

 Glutamate/glutamine

2096.524

658.272

2359.04

878.482

 Ornithine

212.338

85.775

184.849

69.873

 Citrulline

69.446

39.718

75.05

50.441

 Arginine

431.024

182.215

394.565

149.594

Organic acids

 Lactate

22862.683

9246.29

20956.576

8926.553

 Pyruvate

1168.544*

604.675

803.474

539.098

 Succinate

48.143

35.538

41.793

29.968

 Fumarate

70.313

26.403

62.708

25.253

 Malate

521.019

205.905

476.648

198.079

 alphaKetoglutarate

144.24

143.952

113.438

118.669

 Citrate

41.677

33.591

36.853

24.096

Acylcarnitines

 Free carnitine: C0

3369.034

1006.646

3631.978

1243.598

 C2

455.175

288.39

485.702

312.966

 C3

5.206

2.024

5.019

2.018

 C4/Ci4

3.541

4.994

3.008

2.594

 C5:1

1.033

0.397

1.03

0.41

 C5

1.667

1.15

2.246

5.666

 C4OH

2.789

2.231

2.378

1.778

 C6

3.58

3.882

2.956

2.855

 C5OH

0.676

0.363

0.65

0.343

 C3DC

0.793

0.356

0.809

0.292

 C4DC/Ci4DC

2.439

1.424

2.547

1.192

 C8:1

0.531

0.328

0.532

0.252

 C8

0.942

0.904

0.826

0.694

 C5DC

1.528

1.043

1.43

0.727

 C8:1OH/C6:1 DC

0.216

0.129

0.204

0.123

 C6DC/C8OH

0.353

0.239

0.388

0.226

 C10:3

0.067

0.047

0.067

0.034

 C10:2

0.05

0.03

0.063

0.041

 C10:1

0.261

0.253

0.239

0.164

 C10

0.655

0.6

0.58

0.48

 C7DC

0.108

0.079

0.088

0.049

 C8:1 DC

0.087

0.073

0.093

0.051

 C10OH:C8DC

0.305

0.253

0.31

0.21

 C12:2

0.052

0.034

0.052

0.035

 C12:1

0.364

0.281

0.366

0.287

 C12

1.359

1.073

1.31

1.125

 C12:2OH/C10:2 DC

0.075

0.045

0.064

0.04

 C12:1OH/C10:1 DC

0.224

0.178

0.202

0.114

 C12OH/C10DC

0.441

0.472

0.417

0.382

 C14:3

0.078

0.052

0.073

0.054

 C14:2

1.126

1.025

0.902

0.83

 C14:1

2.726

2.354

2.449

2.232

 C14

4.156

3.277

3.781

3.373

 C14:3OH/C12:3 DC

0.032

0.025

0.028

0.022

 C14:2OH/C12:2 DC

0.174

0.121

0.143

0.081

 C14:1OH/C12:1 DC

0.704

0.538

0.701

0.431

 C14OH/C12DC

0.502

0.525

0.487

0.381

 C16:3

0.198

0.164

0.157

0.103

 C16:2

1.533

1.201

1.199

0.948

 C16:1

6.736

5.227

5.751

3.973

 C16

20.041

15.253

17.878

12.497

 C16:3OH/C14:3-DC

0.053

0.038

0.045

0.024

 C16:2OH/C14:2 DC

0.477

0.336

0.412

0.248

 C16:1OH/C14:1 DC

1.306

1.077

1.256

0.834

 C16OH/C14DC

1.18

1.265

1.229

1.059

 C18:3

1.463

0.982

1.354

0.925

 C18:2

20.561

15.909

17.722

13.495

 C18:1

46.521

37.117

40.451

28.311

 C18

11.278

8.401

10.817

8.203

 C18:3OH/C16:3 DC

0.186

0.158

0.168

0.101

 C18:2OH/C16:2 DC

1.357

1.235

1.323

1.177

 C18:1OH/C16:1 DC

2.683

2.889

2.844

2.749

 C18OH/C16DC

0.695

0.68

0.732

0.523

 C20:4

2.023

1.801

1.778

1.872

 C20:3

0.63

0.597

0.57

0.431

 C20:2

0.308

0.271

0.261

0.164

 C20:1

0.554

0.484

0.485

0.409

 C20

0.369

0.4

0.329

0.308

 C20:3OH/C18:3 DC

0.075

0.059

0.074

0.056

 C20:2OH/C18:2 DC

0.053

0.034

0.05

0.03

 C20:1OH/C18:1 DC

0.071

0.062

0.062

0.046

 C20OHC18DC/C22:6

0.212

0.248

0.198

0.206

 C22:5

0.264

0.299

0.247

0.277

 C22:4

0.241

0.279

0.193

0.158

 C22:3

0.064

0.057

0.056

0.044

 C22:2

0.051

0.035

0.044

0.025

 C22:1

0.069

0.05

0.065

0.038

 C22

0.059

0.049

0.062

0.046

  1. Data are shown as means and standard deviations (pmol/mg tissue). Metabolic intermediates were measured in muscle homogenates. Group comparisons between muscle from patients with rheumatoid arthritis and from controls were performed using logarithmically transformed metabolic intermediates and mixed models. Prefix C denotes acylcarnitines followed by carbon number and degree of unsaturation. Suffixes OH and DC denote hydroxyl and dicarboxyl groups, respectively. *P < 0.05 for comparison with matched controls