Fig. 1
BCP crystals activate Syk and PI3K in primary macrophages and DC. a, e Human macrophages and b, f DC (2 × 106 cells/well) were stimulated with BCP crystals (50 μg/ml) for the indicated time points, and phosphorylation of Syk and PI3K was detected by immunoblotting using phospho-specific antibodies. Representative blots of three independent experiments are shown. c, d, g, h Densitometric analysis of three blots was performed using ImageJ software. Bar graphs illustrate the mean (± SEM) increase in phosphorylation, relative to the untreated sample (0) and normalised to total Syk/PI3K protein. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001