Fig. 7

TLR8 and IFN-inducible gene signature in freshly isolated dcSSc monocytes carrying infectious EBV. a PCR products of EBV-lytic gene in freshly isolated monocytes (Mo) and B lymphocytes (B-ly) from two representative diffuse cutaneous systemic sclerosis (dcSSc) patients and one healthy donor (HD); 293 and Raji cells were used as negative and positive controls, respectively. b Heatmap showing the expression of the 156 most significantly upregulated genes (moderated t test) FDR q < 0.25 in freshly isolated lytic/EBV-positive dcSSc compared to HD monocytes. Colors are scaled within each gene so that red and blue indicate expression of values ≥2 standard deviations above and below, respectively, the mean (white) computed across all samples. c Heatmap of the expression of select genes, with colors scaled in the same manner as in panel b. d mRNA expression of indicated genes. Results are expressed as the fold-change normalized to mRNA expression in a single sample from HDs. Levels of 18S ribosomal rRNA were used as an internal control. Bars represent the mean ± SEM. Values were calculated using two-tailed t test