Fig. 2

miR-155 expression requires the NLRP3 inflammasome. a Systemic sclerosis (SSc) lung fibroblasts (n = 7) were treated with 20 μM YVAD for 48 h. miRNA was extracted and the resulting cDNA was assayed for miR-155 levels normalized to SNORD44. b Hydroxyproline was measured in the culture supernatants from a. Statistical analyses for a and b used the Wilcoxon ranked paired t test. c In mouse cells, miR-155 expression was induced with 20 μM bleomycin (Bleo) + 20 μM YVAD for 48 h and analyzed for miR-155 expression normalized to SNORD47. Data are presented as the average from two independent experiments with three replicates (n = 6 for each condition) + SEM. d Hydroxyproline was measured from the culture supernatants from c. Statistical analyses for c and d used the Mann-Whitney t test