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Table 1 Primers for real-time polymerase chain reaction (PCR)

From: RANK/RANKL/OPG system in the intervertebral disc

Genes

Assay IDa

Size (bp)

RANK

Rn04340164_m1

63

RANKL

Rn00589289_m1

69

OPG

Rn00563499_m1

75

IL-1β

Rn00580432_m1

74

MMP-3

Rn00591740_m1

67

MMP-13

Rn01448192_m1

93

18S rRNA

Hs99999901_s1

187

  1. RANK receptor activator of nuclear factor kappa, RANKL rank ligand, OPG osteoprotegerin, MMP matrix metalloproteinase, rRNA ribosomal RNA. aTaqMan Gene Expression Assays (Applied Biosystems)