Fig. 3

Whole body inducible Wnt5a knockout (Wnt5a cKO) mice have reduced osteoclast activity after induction of arthritis. a Tartrate-resistant acid phosphatase (TRAP) staining, indicated by purple color in control (left panel) and Wnt5a cKO (right panel), indicates fewer areas of osteoclast activity in the Wnt5a cKO mice at day 7. b Quantification of the percent of TRAP-positive area revealed reduced osteoclast activity in the Wnt5a cKO (white bars) compared to the controls (gray bars). c The levels of mRNA for the TRAP, Cathepsin K (CTSK), macrophage colony-stimulating factor (MCSF), matrix metalloproteinase 2 (MMP2) and MMP9 were reduced in the paws from the arthritic Wnt5a cKO mice compared to the arthritic controls. *P ≤ 0.05. A trend toward reduced RANKL transcript was noted