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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody

Fig. 2

Suppression of T-cell activation by Dow2 in vitro. a T-cell proliferation was assessed following mixed lymphocyte reactions (MLR) involving carboxyfluorescein succinimidyl ester (CFSE)-labeled C57BL/6JJcl splenocytes cocultured with irradiated (20 Gy) BALB/c splenocytes in the presence of Dow2, 17A2, or isotype control (1 μg/ml). Each panel is annotated with the number of CFSE-positive cells and, in parentheses, the concentration of IFN-γ (ng/ml) in coculture supernatants as determined by ELISA. b Left panel: IFN-γ production by pan-T cells following incubation with Dow2, 17A2, Dow2 + 17A2, or rat IgG (1 μg/ml). c Left panel: IL-17 production by T cells following incubation with Dow2, 17A2, Dow2 + 17A2, or rat IgG. Results of triplicate samples are presented as the mean ± SEM. Right panels: IFN-γ (b) or IL-17 (c) production by pan-T cells following incubation with Dow2 (0–1000 ng/ml) and/or 17A2 (0–1000 ng/ml). Representative data from three independent experiments with similar results are shown. *P < 0.05, **P < 0.005

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