Fig. 3
From: The impact of age and gut microbiota on Th17 and Tfh cells in K/BxN autoimmune arthritis
Middle-aged naïve T cells display impaired T helper 17 (Th17) cell differentiation. a Representative plots of T cell receptor (TCR) Vβ6 vs. TCR Vβ14 expression in splenic Th17 cells from segmented filamentous bacteria-negative (SFB–) and SFB+ middle-aged K/BxN mice. The quantitative data show the percentage of skewed Vβ14+Vβ6+ usage are calculated by 100 × [Vβ6+Vβ14+ Th17 cell percentage ⁄total (both Vβ14- and Vβ14+) TCR Vβ6+ Th17 cell percentage]. The quantitative data on TCR skewing in the lung and small intestine-lamina propria (SI-LP) are also shown (n = 3–6 mice in each group). b Fluorescence-activated cell-sorted splenic naïve CD4+ T cells from SFB– young and middle-aged K/BxN mice were in vitro cultured under Th17 polarization conditions for 4 days. Representative plots and quantitative data on Th17 polarization are shown (n = 4–7 mice in each group). c Representative plots and quantitative data on IL-23 expression in the spleen of SFB+ young and middle-aged K/BxN mice (n = 3 mice in each group)