Fig. 4

Impact of IL-15 stimulation on IL-17 production and on Th17 differentiation. BMMC from OA (right panel) and RA (left panel) patients cultured for 72 hours without (control) or with addition of IL-15 (25 ng/ml). At the end of cell culture, supernatants were collected for ELISA (a) and cells were designated for FACS analysis (b, c). a Concentration of secreted IL-17A in cell culture supernatants (RA n = 11; OA n = 11). b Percentage of IL-17A-expressing CD3⁺CD4⁺ cells out of total CD3⁺CD4⁺ cells (RA n = 11; OA n = 13).c Intracellular amount of IL-17A (reflected by MFI) in CD3⁺CD4⁺ cells (RA n = 11; OA n = 13). IL interleukin, MFI mean fluorescence intensity, OA osteoarthritis, RA rheumatoid arthritis