Fig. 1

BANK1 expression in CIA. a BANK1 mRNA levels were detected by real-time PCR. b The percentage of BANK1⁺CD19⁺ cells was detected by flow cytometry. c The BANK1 protein level was evaluated by western blotting (WB), and the relative density is shown. d BANK1 expression in draining lymph nodes (LNs) in naive DBA1/J mice and collagen-induced arthritis (CIA) mice (14 and 35 days after immunization) was observed by immunohistochemical staining. BANK1 expression in lymphoid nodules in LNs from naïve DBA1/J mice and the swollen LNs from CIA mice 14 and 35 days (d) after immunization (original magnification × 20 and × 40). Representative data from one of three independent experiments are shown (c, d). e Correlation between BANK1 mRNA levels and clinical scores and also the titers of three anti-collagen type II (anti-CII) antibodies was analyzed. The results represent pooled data from three independent experiments. Bars show the means ± SEM. The post-hoc comparisons were done following one-way analysis of variance for repetitive testing. Correlation was analyzed using Spearman’s test. Linear regression analysis was performed when correlation was identified: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001