Fig. 2

Immunohistochemical analysis of tissues and cells using anti-3S-P antibodies. a Expression of anti-3S-P target antigens in RA-sSS synovial tissue and salivary tissue. RA-sSS synovium was stained using antibodies against KLH and R-anti-3S-P. RA-sSS salivary gland tissue was stained using R-anti-3S-P and anti-KLH as control (original magnification × 400). b Confocal immunofluorescence microscopic images of FLSs reacted with R-anti-3S-P or anti-KLH antibody. Antibody staining (FITC, green), and nuclear staining (DAPI, blue) are shown. Scale bar represents 20 μm. 3S-P Secondary Sjögren’s syndrome-associated peptide, DAPI 4′6-Diamidino-2-phenylindole, FITC Fluorescein isothiocyanate, FLS Fibroblast-like synoviocyte, KLH Keyhole limpet hemocyanin, RA Rheumatoid arthritis, R-anti-3S-P Rabbit-anti-secondary Sjögren’s syndrome-associated peptide, sSS Secondary Sjögren’s syndrome