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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome

Fig. 5

Identification of vimentin as the target autoantigen of anti-3S-P autoantibodies. a Autoantigens of anti-3S-P and vimentin are present in immunoprecipitates (IP) obtained by using antivimentin antibody from lysates of FLSs, whereas no band was seen in negative controls. b ELISA data for the detection of recombinant human vimentin using the R-anti-3S-P antibody diluted 1:50 to 1:800. The anti-KLH antibody was used as an isotype control and was diluted 1:50 to 1:800. c Western blot (WB) of recombinant human vimentin probed with antivimentin, R-anti-3S-P, and anti-KLH antibodies. ELISA Enzyme-linked immunosorbent assay, FLS Fibroblast-like synoviocyte, KLH Keyhole limpet hemocyanin, R-anti-3S-P Rabbit-anti-secondary Sjögren’s syndrome-associated peptide, SDS Sodium dodecyl sulfate

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