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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Soluble uric acid increases PDZK1 and ABCG2 expression in human intestinal cell lines via the TLR4-NLRP3 inflammasome and PI3K/Akt signaling pathway

Fig. 1

PDZK1 and ABCG2 expression mediated by stimulation with soluble urate in HT-29 and Caco-2 cells. Cells treated with soluble urate or 10 mM NaOH for 24 h. a Relative mRNA levels of PDZK1 and ABCG2 determined by RT-qPCR. Data presented as mean ± standard error of the mean (SEM). *P < 0.05 and **P < 0.01, compared to control cells; n = 3. b Representative western blots of PDZK1 and ABCG2. Protein expression normalized to that of GAPDH. c Efflux function of ABCG2 evaluated by detecting the intracellular fluorescence of e-Fluxx-ID® Green Dye with or without the ABCG2 inhibitor, novobiocin. Tinted histograms show fluorescence of untreated cells, and nontinted histograms show fluorescence of inhibitor-treated cells. Multidrug resistance activity factor (MAF) value is indicative of corresponding protein activity. ABCG2 ATP-binding cassette transporter, subfamily G, member 2, PDZK1 PDZ domain containing 1, UA uric acid

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