Fig. 2

Friend leukemia virus integration 1 (Fli1) regulates the expression of interleukin (IL)-33 in dermal fibroblasts. a, b Evaluation of IL-33 expression in skin from wild-type (WT) and Fli1^+/− mice without any treatment by qRT-PCR (a, n = 10) and immunohistochemistry (b, n = 5; original magnification ×400, scale bar = 50 μm). High-power field images spotlighting dermal fibroblasts are in the rightmost column. c The result of immunostaining without anti-IL-33 antibody in the skin of WT mice under a physiological condition. d, e Evaluation of IL-33 expression in dermal fibroblasts isolated from WT and Fli1^+/− mice by qRT-PCR (d, n = 10) and immunoblotting (e, n = 4). f, g Results of the chromatin immunoprecipitation assay regarding Fli1 occupation on the IL33 promoter in human dermal fibroblasts without any treatment (f, n = 4) and those cells treated with IL-1β or tumor necrosis factor (TNF)-α for 24 h (g, n = 4). For a and c, messenger RNA (mRNA) levels of the Il33 gene were normalized to those of the Gapdh gene. For f, the occupancy of the Il33 promoter by Fli1 was quantified with qRT-PCR. For b, d, and e, representative results are shown. In each graph, the relative value compared with the control group is expressed as mean ± SEM. AU Arbitrary units, IgG Immunoglobulin G, TNF-α Tumor necrosis factor-α