Fig. 3

Representative flow cytometric profiles of spleen cells (a), peripheral leukocytes (b), and peripheral CD115⁺ monocytes (c) in untreated and anti-mouse CD11b monoclonal antibody (5C6)-treated FcγRIIB-deficient mouse strain (KO1) mice at 10 months of age. a Spleen cells were stained with fluorescent-labeled CD69/B220, peanut agglutinin (PNA)/B220, and CD138/B220. The frequencies of CD69⁺ activated B cells and of PNA⁺ germinal center B cells per total B cells are shown as squares. The frequencies of CD138⁺ plasma cells and B220⁺ B cells per total cells are shown as circles. b Peripheral leukocytes were stained with fluorescent-labeled Gr-1/CD11b. The frequencies of neutrophils, monocytes of Gr-1⁺ and Gr-1^- subsets per total cell are shown as squares. c Peripheral leukocytes were stained with fluorescent-labeled CD115/Gr-1/CD11b. Gr-1/CD11b expression profiles of CD115⁺ gated monocytes clearly show Gr-1⁺ and Gr-1^- monocyte subsets