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Table 2 Spleen weight and frequencies of spleen cell subpopulations and peripheral leukocyte subsets in untreated, normal rat IgG-treated, and 5C6-treated KO1 micea

From: Anti-CD11b antibody treatment suppresses the osteoclast generation, inflammatory cell infiltration, and autoantibody production in arthritis-prone FcγRIIB-deficient mice

  Treatment
None Rat IgG 5C6
Spleen weight (g) 0.20 ± 0.05 0.19 ± 0.04 0.19 ± 0.02
Spleen cell populations (%)
 B220+B cells/total cells 48.0 ± 2.7 47.7 ± 3.3 56.4 ± 1.9b
 CD69+B220+ B cells/total B cells 5.3 ± 1.9 4.5 ± 1.0 2.7 ± 0.2b
 PNA+B220+ B cells/total B cells 3.8 ± 1.2 3.9 ± 0.5 1.2 ± 0.1c
 CD138+plasma cells/total cells 2.1 ± 0.5 1.7 ± 0.2 1.1 ± 0.2d
 CD4+T cells/total cells 17.5 ± 0.4 16.2 ± 0.8 17.3 ± 0.7
 CD69+CD4+ T cells/total T cells 35.9 ± 4.2 29.9 ± 2.5 34.64 ± 1.53
 CD11b+ cells/total cells 12.0 ± 2.5 ND 9.1 ± 1.5
 CD11c+ cells/total cells 6.9 ± 1.3 ND 5.8 ± 0.6
Peripheral blood (%)
 Neutrophils/total cells 16.8 ± 1.7 ND 9.5 ± 2.4
 Monocytes/total cells 54.0 ± 2.3 50.5 ± 11.9 41.3 ± 1.8b
 Gr-1+ monocytes/total cells 6.2 ± 1.4 ND 4.4 ± 0.6
 Gr-1- monocytes/total cells 47.8 ± 1.7 ND 37.0 ± 1.8e
  1. ND not defined, 5C6 anti-mouse CD11b monoclonal antibody, KO1 FcγRIIB-deficient mouse strain
  2. aValues are the mean ± SEM of at least 6 female mice aged 10 months
  3. bDifferences were statistically significant versus untreated KO1 and versus normal rat IgG-treated KO1 (analysis of variance (ANOVA) P < 0.05)
  4. cDifferences were statistically significant versus untreated KO1 and versus normal rat IgG-treated KO1 (ANOVA P < 0.01)
  5. dDifferences were statistically significant versus untreated KO1 (ANOVA P < 0.01) and versus normal rat IgG-treated KO1 (ANOVA P < 0.05)
  6. eDifference was statistically significant versus untreated KO1 (Student’s t test P < 0.05)