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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Mice with miR-146a deficiency develop severe gouty arthritis via dysregulation of TRAF 6, IRAK 1 and NALP3 inflammasome

Fig. 1

MiR-146a participates in monosodium urate (MSU)-induced inflammation a. The purity of bone-marrow-derived macrophages (BMDMs). Bone marrow cells of miR-146a knockout (KO) and wild-type (WT) B6 mice were cultured in RPMI-1640 medium containing 30 ng/mL macrophage colony-stimulating factor (M-CSF) for 7–9 days, and then co-stained with anti-F4/80 and anti-CD11b. The double-positive cells as the percentage of BMDMs among all cells is indicated (n = 3).b Relative expression of miR-146a following culture with 0.25 mg/mL MSU crystals for 0, 4, and 8 h. c The change in the two macrophage subsets. A total of 3 mg/0.5 mL MSU was injected into the peritoneal cavity; after 0, 2.5, and 5 h, peritoneal exudate cells were harvested and stained with anti-F4/80, and anti-CD11b, F4/80 + CD11bhigh, and F4/80 + CD11bintmacrophage subsets were sorted: n = 3 for each group; groups were compared using the t test. d Relative expression of miR-146a in the sorted macrophages of the 3 mg/0.5 mL MSU-induced peritonitis model. ud, undetected

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