Fig. 4

Targeting IL-17A delays osteogenic differentiation of AS BdCs. Ct and AS-BdCs were stimulated with or without IL-17A blockade (10 μg/mL) in the presence of AS serum (1/10 dilution) in osteogenic medium. On the indicated day, osteogenic differentiation was assessed by (a) ALP (upper) and ARS (lower) staining, (b) ALP activity assays. The stimulated cells for 7 days were subjected to (c) immunoblotting of the indicated proteins and (d) quantitative RT-PCR of the indicated genes. Representative data are shown (n = 6). One-way ANOVA analysis was performed to determine statistical significance. Data are presented as means ± SDs. N.S., not significant; *P < 0.05 indicates significant differences between the two groups