Fig. 2

Smoking-induced arthritis aggravation and increase in Th17 activity are AHR-dependent. a Ahr and Cyp1a1 mRNA expression in DLNs from non-immunized (Naïve), sham-immunized (Sham) and Immunized mice (AIA) 7 days after first immunization. Mean ± SEM, n = 4/group, ^**P < 0.01 by one-way ANOVA followed by Bonferroni post hoc test. b Cyp1a1 mRNA expression in DLNs of AIA mice 6 h after exposure to cigarette smoke (two cigarettes, day 12 after immunization). Mean ± SEM, n = 4 mice/group. ^*P < 0.05, one-way ANOVA followed by Bonferroni post hoc test. c AIA mice were exposed to cigarette smoke and treated with CH223191. Number of neutrophils in the joints and articular hyperalgesia were determined. Mean ± SEM, n = 4/group, ^**P < 0.01, ^***P < 0.001, one-way ANOVA followed by Bonferroni post hoc est. d Th17 frequency in DLNs of AIA mice exposed to cigarette smoke and treated with CH22319. Mean ± SEM, n = 4/group, ^*P < 0.05, one-way ANOVA followed by Bonferroni post hoc test. e Ahr^−/− or WT mice during AIA were exposed to cigarette smoke. Mean ± SEM, n = 4/group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, one-way ANOVA followed by Bonferroni post hoc test. f Frequency of Th17 cell in the DLNs of WT and Ahr^−/− mice exposed to cigarette smoke. Mean ± SEM, n = 4/group. ^*P < 0.05, ^**P < 0.01, one-way ANOVA followed by Bonferroni post hoc test. Data are representative of at least two independent experiments. CH223191 1-Methyl-N-[2-methyl-4-[2-(2-methylphenyl)diazenyl]phenyl-1H-pyrazole-5-carboxamide, DLN draining lymph nodes, IL-17 interleukin 17