Fig. 3
From: Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
Surface and total expression of CXCR5, CCR6 and CXCR4 in B lymphocytes from PB and SF of patients with RA. Mononuclear cells isolated from PB and SF labeled with a combination of two fluorochrome-conjugated mAbs and analyzed by flow cytometry. Surface (nonpermeabilized) and total (permeabilized) expression of chemokine receptors studied in CD20+ cells from PB and SF of RA patients. Data expressed as mean ± SE of rMFI from five independent experiments compared to PB expression of each chemokine receptor, considered 100%. Representative flow cytometry histograms showing surface expression of CXCR5, CCR6 and CXCR4 in CD20+ cells from PB and SF of a patient with RA. Dotted histograms represent isotype-matching control; dashed and closed histograms represent expression in B cells from PB and SF, respectively. **p < 0.01, t test for paired samples. rMFI relative mean fluorescence intensity, PB peripheral blood, SF synovial fluid