Table 2 Staining matrix showing antibodies and their corresponding fluorochrome conjugates measured in ten separate staining tubes
Fluorochrome | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | T10 |
|---|---|---|---|---|---|---|---|---|---|---|
Pacific Blue | CD3 | CD3 | CD3 | CD3 | CD3 | CD3 | CD3 | CD3 | CD3 | CD3 |
FITC | CD27 | CD64 | CD244 | CD35 | CD46 | CD45RA | BDCA2 | CD138 | CD134 | CD28 |
PE | IgD/ CD14/ CD56 | CD33/ NKG2D | CD163/ CRTH-2 | CD119 | CD88 | CXCR4 | CD1c | CD38 | ICOS | CD31 |
PE-Cy5 | CD45RA | HLA-DR | CD128b | CD107a | CD21 | CD62L | HLA-DR | HLA-DR | CD154 | CD45RA |
PE-Cy7 | CD8 | CD56/ CD14 | CD14 | CD14 | CD14 | CCR7 | CD14 | CD69 | CD69 | CD69 |
APC | CD19 | CD32 | CCR2 | CD120b | CD55 | CXCR3 | CD11c | CD20 | CD25 | CD152 |
APC-Cy7 | CD4/ CD16 | CD4/ CD16 | CD4/ CD16 | CD4/ CD16 | CD4/ CD16 | CD4/ CD16 | CD19 | CD19 | CD4/ CD16 | CD4/ CD16 |