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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA

Fig. 1

Downregulation of mPR3 after treating neutrophils with PECAM-1. a interaction between platelet endothelial cell adhesion molecule-1 (PECAM-1) and CD177 at various concentrations. b Representative histogram of the effect of PECAM-1 on membrane-bound proteinase-3 (mPR3) expression in a dose-dependent manner. c Incubation of TNF-α-primed neutrophils with PECAM-1 at 30 μg/ml significantly decreased mPR3 expression. Bars denote means ± SD of mPR3 expression (mean fluorescence intensity; MFI). d Incubation of TNF-α-primed neutrophils with PECAM-1 at 30 μg/ml significantly increased proteinase-3 (PR3) levels in the supernatant. Bars denote means ± SD of PR3 concentration (ng/ml). Neutrophils treated with phorbol myristate acetate (PMA) were employed as positive control. *p < 0.05. FACS fluorescence-activated cell sorting, JAM-1 junctional adhesion molecule-1, OD optical density

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Arthritis Research & Therapy

ISSN: 1478-6362

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