Fig. 5

Anti-arthritic activity of monoclonal antibodies (mAbs) KH4–8 and KH7–33 in a mouse model of collagen-induced arthritis. a arthritis index, b squeaking score (a value of 0 represents no indication of pain), c paw volume, and (d) body weight, indicating the severity of arthritis in mouse limbs. The number of arthritic limbs was quantitated, and each limb was assigned a severity score of 0–4. Data indicate the number of arthritic limbs per arthritic mouse and the mean severity score of each arthritic limb. The black arrows indicate the starting day (day 15) of mAb or prednisolone administration. NOR (normal), n = 8; CON (negative control), KH4–8, n = 8; KH7–33, n = 8; PRE (prednisolone, positive control), n = 8. Values are expressed as mean ± standard error of the mean. ^###P <0.001 versus the NOR group and *P <0.05, **P <0.01, ***P <0.001 versus the CON group (two-way analysis of variance followed by Bonferroni correction)