Fig. 6

GTS-21 had no effect on the expression of CD80 and major histocompatibility complex II (MHC II) on the surface of dendritic cells (DCs) during DC maturation. On day 6 of bone marrow–derived dendritic cell culture, immature DCs were stimulated with GTS-21 (0.1, 1, 10, or 100 μmol/L) for 24 h. The expression levels of MHC II and CD80 were detected by using flow cytometry. All data shown were gated on CD11c⁺ F4/80⁻ cells. a, c Histograms of MHC II and CD80 in the CD11c⁺ F4/80⁻ cells are shown. b, d The changes of mean fluorescence intensity (MFI) of MHC II and CD80 were analyzed. Data are expressed as the mean ± standard deviation. All data are representative of three independent experiments. Abbreviation: ctrl control