Fig. 5

Enhanced response of Col6a1-Bma1^−/− mice to collagen antibody-induced arthritis. a Hind paw thickness and disease score after initiation of collagen antibody-induced arthritis (CAIA) in wild-type (n = 4) and Col6a1-Bmal1^−/− (n = 5) mice. One-way analysis of variance (ANOVA) and Bonferroni multiple comparisons. b Serum IL-6 levels in CAIA animals at the time they were killed on day 7. Analysis by t test. c Quantification of leukocytes within hind paws of arthritic (n = 4–5/group) and non-arthritic (n = 4/group) animals. Two-way ANOVA and post hoc Bonferroni correction. Stars directly above CAIA bars indicate significant difference compared with control counterparts. d Representative plots showing increased presence of LycC^hi monocytes in inflamed limbs of Col6a1-Bmal1^−/− mice, and quantification of Ly6C^hi and Ly6C^lo monocytes within hind paws of arthritic (n = 4–5/group) and non-arthritic (n = 4/group) animals. Two-way ANOVA and post hoc Bonferroni correction. Stars directly above CAIA bars indicate significant differences compared with control counterparts. e Transcripts of inflammatory cytokines in hind limbs harvested from control (n = 4/group) and CAIA mice (n = 4–5/group). Two-way ANOVA and post hoc Bonferroni correction. Stars directly above CAIA bars indicate significant difference compared with control counterparts