Fig. 1

Renal bradykinin 1 receptor (B1R) and B2R expression in MRL/lpr mice and B6 mice as assessed by western blot analyses. Rabbit anti-mouse B1R antibody (Bioss Inc., Woburn, MA, USA), rabbit anti-mouse B2R antibody (Bioss Inc.), and anti-α-tubulin (Cell Signaling Technology, Beverly, MA, USA) were used as the primary antibodies. Horseradish peroxidase (HRP)-conjugated secondary antibodies and the ECL-plus detection kit (Amersham, Little Chalfont, UK) were used for western blot. Western blot analysis revealed that renal B1R expression was increased in MRL/lpr mice compared with B6 mice (a, c) but that B2R expression was decreased in MRL/lpr mice compared with B6 mice (b, d). Shown data are representative of blots from five B6 mice and five MRL/lpr mice