Fig. 4

In vitro proliferative response of CD19⁺ B cells and CD4⁺ T cells from B10.RIIIS/J-Eae39r2 sub-congenic mice. ³H-thymidine incorporation, represented as cpm ± SEM, of CD19⁺ B cells following in vitro stimulation with titrated concentrations (0–40 μg/ml) of anti-IgM antibody (a); titrated concentrations (0–10 μg/ml) of lipopolysaccharide LPS (b); combination of fixed concentration (10 ng/ml) of IL-4 and titrated concentrations (0–4 μg/ml) of anti-CD40 antibody (c). d ³H-thymidine incorporation of CD4⁺ T cells following in vitro stimulation with combination of titrated concentrations (0–3 μg/ml) of anti-CD3 antibody and fixed concentration of anti-CD28 antibody (3 μg/ml). e IL-2 production (mean ± SEM) in the supernatant of CD4⁺ T cells stimulated with anti-CD3/CD28 antibodies. Statistical analyses were performed using the Mann-Whitney U test; *p < 0.05