Fig. 5

Quantitative analysis of active TBX3 in nuclear lysates of CD19⁺ B cells from B10.RIII mice. a Comparison of active TBX3 levels in CD19⁺ B cells isolated from naïve B10.RIII mice (n = 4), with and without in vitro stimulation with a fixed concentration (10 μg/ml) of anti-IgM antibody for 48 h. b, c Collagen-induced arthritis (CIA) development and corresponding TBX3 activity in B10.RIII mice whereby four mice were killed at indicated time points after collagen type II (CII) immunization and levels of active TBX3 were determined with and without ex vivo re-stimulation with fixed concentration (10 μg/ml) of anti-IgM antibody for 48 h. Statistical analyses were performed using the Mann-Whitney U test; *significant (p < 0.05) compared to day 0 unstimulated samples; ^#significant compared to day 7 unstimulated samples; ^§significant compared to respective unstimulated samples