Fig. 1

RvD1 does not impair RAW264.7 cell viability. RAW264.7 cells were incubated with different concentrations of RvD1 (0–500 nM) without or with 50 ng/ml LPS for 72 h. Cell viability percentages were obtained by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-SH-tetrazolium bromide assay (a), and LDH release was measured in cell supernatants by a commercial kit (b). Cell death and LDH release were induced by the addition of 0.5 mM H₂O₂. Beclin-1 protein expression was assessed in cell extracts by western blot (c). ANOVA test was performed to compare each condition. Results are expressed as mean ± SEM for n = 4. Data are means ± SEM, and one-way ANOVA was performed to compare the results. ^##p < 0.01 compared to untreated cells