Fig. 4

miR-191 regulates C/EBPβ expression in RA-FLS. a quantitative RT-PCR analyses demonstrated the decrease of mRNA levels of C/EBPβ in RA-FLS after transfected with miR-191-mimic for 24 h. b Western blot analyses demonstrated the decrease of C/EBPβ protein level in RA-FLS after transfected with miR-191-mimic for 48 h. c Sequences of the predicted miR-191 target sequences in the 3′-UTR of C/EBPβ and its mutant containing altered nucleotides in the 3′-UTR. d Luciferase reporter assays demonstrated the inhibition of C/EBPβ 3′-UTR by direct interaction with miR-191. All experiments were repeated three times. Data were presented as mean ± SEM (n = 3, *p < 0.05 by t test)