Fig. 5
C/EBPβ mediated the miR-191 regulation of cell proliferation. a Western blot analyses demonstrated the knockdown of C/EBPβ in RA-FLS after transfection of siRNAs for 48 h. b RA-FLS transfected with NC or si-C/EBPβ2 were plated at 4 × 103 cells/well in 96-well plates; the cell viability of RA-FLS was determined by CCK8 at 0, 36, and 72 h. c Cell cycle analyses showing the increased G1/S transition after knockdown of C/EBPβ for 48 h. d Western blot analysis of the levels of C/EBPβ in RA-FLS after being overexpressed with C/EBPβ for 48 h. e CCK8 assay showed that overexpression of C/EBPβ rescued the cell proliferation regulation by miR-191 in RA-FLS. All experiments were repeated three times. Data were presented as mean ± SEM (n = 3, **p < 0.01, *p < 0.05 by t test)